Biological Process Development Facility
directed by Wallace Buchholz
http://bpdf.unl.edu/
a member since 7/8/2014

Contact Info

For information contact
Wallace Buchholz.

402-472-2342
University of Nebraska-Lincoln
304 Othmer Hall
820 N. 16th St
Lincoln, NE 68588-0668

USA

Services Provided

This facility provides research grade material and offers production support to both Academics and Companies.

Biologics Produced

  • working cell banks
  • Vaccines
  • Vaccines
  • recombinant proteins from bacteria and yeast
  • Recombinant Proteins
  • full GMP facility. Master cell banks
  • Bacteria
  • Active Pharmaceutical Ingredients

Vector Support

  • Yeast and bacterial systems
  • Saccharomyces
  • Pichia pastoris and E. coli
  • other bacteria

About this facility

The University of Nebraska-Lincoln Biological Process Development Facility (UNL BPDF) uses a synergistic multi-disciplinary approach to advance research-derived candidate vaccines and bio-therapeutics to Phase I/II clinical trials. BPDF research on fermentation processes is focused on modeling, optimizing, and controlling high cell density fermentations of Pichia pastoris, Saccharomyces cerevisiae, and E. coli for production of recombinant proteins. For over 12 years, we have provided customers with access to experienced biopharmaceutical process research & development scientists and engineers, state-of-the-art process development capabilities, and cGMP manufacturing facilities.

The UNL BPDF is equipped with a state-of-the-art cGMP facility with 6,000 ft2 of modular clean rooms and 7,000 ft2 of support space, including a pure steam generator, a water-for-injection (WFI) condenser, a 1000 gal WFI storage tank, and ambient and hot WFI distribution loops. The cGMP facility has a bioreactor capacity of 150 L (working volume), and is able to accommodate a 1000 L fermentor. We are equipped for downstream processing of both secreted and intracellular products derived from yeast or bacteria. This facility is designed to produce Bulk Drug Substances. We also produce Master Cell Banks and Working Cell Banks.